Specific Localization of /J-D-Glucoside Conjugates of 2,4-Dichlorophenoxyacetic Acid in Soybean Vacuoles
نویسنده
چکیده
2,4-Dichlorophenoxyacetic Acid, Glycine max L., Plant Cell Suspension Culture, Vacuole, /?-ü-Glucosides Cell suspension cultures of soybean (Glycine m ax L.) were incubated with [14C]-2,4dichlorophenoxyacetic acid (62 h, 27 °C). The cells were converted to protoplasts by use of the cell-wall degrading enzymes, Driselase and pectinase. This procedure resulted in the release of the cellular amino acid conjugates as well as part of the free 2,4-dichlorophenoxyacetic acid and of the ß-Dglucoside conjugates. A vacuole fraction was obtained by mild osmotic shock in 10% yield, and was characterized by its polypeptide composition. The purified vacuoles were enriched in /?-D-glucoside conjugates, free 2,4-dichlorophenoxyacetic acid being only a minor vacuolar constituent, and amino acid conjugates not being detectable.
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